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Thursday, 17 February 2011 00:00 |
 Tubes for Bullet Blender®
For best results, use tubes that have been verified for use with the Bullet Blender®
- Eppendorf ®Safe-Lock™ snap-cap tubes for BBX24
- Axygen® brand 1.5ml screw-cap tubes for BBX24
 Bullet_Blender_Info__FAQ.pdf 
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Wednesday, 01 December 2010 00:00 |
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| The BULLET BLENDER™ enables you to homogenize, disrupt, or lyse 24 tissue or cell culture samples at a time. Load the samples into polypropylene microcentrifuge tubes, then place them in the BULLET BLENDER™.The "bullets" in the instrument vigorously strike all the sample tubes while in operation. The Bullet Blender™ is now available with Air Cooling™ to preserve your sample quality. |
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Genomic DNA samples in agarose gel stained with ethidium bromide
Lanes 1-4 = Tobacco leaf DNA
 = phage Hind II digest
Lanes 5-8=Rice leaf DNA samples
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Total RNA gel. RNA Integrity Number = 8.5
(thanks to Madhu Prasad @ U. Michigan) |
SDS-PAGE gel stained with Coomassie Blue
wc = whole cell
sf = soluble fraction
Left two lanes control Right two lanes induced |
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Read more...
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Bead table & selection guide |
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Saturday, 21 November 2009 22:51 |
Bead_Selection_Guide.pdf
| Bead | Diameter (mm) |
Density
(mg / microliter)
| Weight (mg) | Beads per 100mg | 100 microliters* of beads (mg) |
| Glass |
0.10
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2.5
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0.0013
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76000
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175
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| Glass |
0.50
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2.5
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0.16
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610
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175
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| Glass |
1.00
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2.5
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1.3
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76
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175
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| Zirconium Silicate |
0.50
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3.8
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0.25
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400
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266
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| Zirconium Silicate |
1.00
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3.8
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2.0
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50
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266
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| Zirconium Silicate |
2.00
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3.8
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16
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6.3
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266
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| Zirconium Oxide |
0.15
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5.5
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0.0097
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10000
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385
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| Zirconium Oxide |
0.50
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5.5
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0.36
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280
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385
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| Zirconium Oxide |
1.00
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5.5
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2.9
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35
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385
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| Zirconium Oxide |
2.00
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5.5
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23
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4.3
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385
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| Stainless Steel |
0.20
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7.9
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0.033
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3000
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553
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| Stainless Steel |
0.50
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7.9
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0.52
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190
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553
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| Stainless Steel |
1.60
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7.9
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17
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5.9
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553
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| Stainless Steel |
3.20
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7.9
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140
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0.74
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553
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| Stainless Steel |
4.80
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7.9
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460
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0.22
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553
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Monday, 26 October 2009 17:14 |
Since the bead amounts are based off of a mass ratio, we have provided a simple way to know how many beads you will need to obtain a certain mass. Our bead tables table is below, and a version complete with a bead calculator is available for download inOpenOffice Calc and Microsoft Excel formats.
Useful estimation - a full scoop with the bead spatula will hold about 50 microliters of 1mm or smaller beads, and you can often approximate the appropriate amount of beads by volume by using a 2:1 sample-to-bead volume ratio. Please know that scoop size may vary with different sized beads or because of human error, and the amount of beads required may change based on the protocol. If you find that measuring by volume causes inconsistency, then go back to measuring by mass. |
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