ExpressArt FFPE Clear Quick RNAready kit
RNA isolations from archival FFPE tissues with No paraffin removal step!
ExpressArt FFPE RNAready overcomes the limitations associated with RNA degradation and interference from RNA cross-linking in FFPE tissues.
Thanks to "FFPE Clear" - a formulation of non-toxic, non-odorous organic solvents, the step of manual paraffin removal is no longer needed !
When working with stored materials, researchers often need to isolate RNA from samples that have been stored as FFPE tissue blocks. These FFPE tissues are a valuable source of information for retrospective research. Although the tissue structure of FFPE samples will have been maintained for histological analysis, damage to the nucleic acid may have occurred through the fixation, embedding, and storage processes impeding measurements of gene expression levels. The degree of RNA fragmentation that typically has already occurred in FFPE tissues cannot be reversed, so researchers are faced with the challenge of optimizing RNA gene expression analysis protocols when working with these samples.ExpressArt FFPE RNAready overcomes the limitations associated with RNA degradation and interference from RNA cross-linking in FFPE tissues. The FFPE RNAready kit efficiently isolates and preserves mRNA utilizing a specially developed lysis solution and an innovative universal inhibitor of RNases that displaces tightly bound RNA from high-molecular-weight aggregates. Subsequent treatment with a unique de-modification reagent reverses formalin induced cross-links, resulting in total RNA optimized for reverse transcription reactions and subsequent downstream applications.
FFPE RNAready together with ExpressArt RNA amplification TRinucleotide primer technology is now available to amplify microgram quantities of high quality RNA from very small amounts of degraded total RNA, with minimal loss of sequence.
Large Samples:
Up to five sections (10 to 20 µm thick), or up to 30 mg of unsectioned core samples, per reaction. Typical yields of ~ 2 µg RNA per slide (10 µm thick, ~ 1 cm2 tissue area).
Small Samples:
Laser microdissected FFPE samples result in yields of ~ 2 to 7 pg/cell (0.2 to 0.7 ng RNA from 100 micro dissected cells.
Expressart-FFPE-Clear-quick_protocol.pdf
FFPE_Quick_RNAready_kit_02-2011.pdf
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