innuCONVERT Bisulfite All-In-One Kit

The innuCONVERT Bisulfite All-In-One Kit al-lows researchers to extract DNA from a variety of relevant starting materials and then completely convert non-methylated cytosine to uracil in a few hours.

A specially optimized universal buffer can be used for processing different lysis protocols, while DNA sample/lysate denaturation and bisulfite treatment take place in a single reaction vessel. This is followed by on-column purification and desulfonation of the converted DNA. The pure nucleic acid obtained can then be easily used for later downstream applications (such as PCR, sequencing).

Starting material:

  • ƒƒPurified DNA samples (500 pg – 10 μg)
  • FFPE tissue sections (formalin-fixed, paraffin-embedded, 1-3 sections, 10 μm each)
  • FFPE tissue plugs (formalin-fixed, paraffin-embedded, 10 mg)
  • Cell lines (no more than 5 x 105 cells)
  • Bronchial aspirates, swabs, peritoneal cavity fluid, pleural effusions, sputum
  • Fresh tissue (no more than 1 mg)

Average yield:

Depends on the sample and the amount used

Average purity:

1.8 – 2.0

Extraction time:

  • Lysis: approx. 3 hours or more
  • Conversion reaction: 45 minutes
  • Purification and desulfonation: 45 minutes