Tips for Detecting Phosphoproteins
- Published: Thursday, 11 July 2019 12:07
Protein phosphorylation is very important for protein post-translational modification that controls many cellular processes including metabolism, transcriptional and translation regulation, degradation of proteins, cellular signaling and communication, proliferation, differentiation, and cell survival [1].
Approximately 35% of human proteins are phosphorylated. Phosphoproteins are low in abundance, and, therefore, are, sometimes, challenging to detect and characterize by Western blot and other methods. Western blot is the most common method for detecting and quantifying phosphorylated proteins.