"...limitations exist in the collection and analysis of all Western blot data, regardless of the detection method ..
Tus, only with proper quality control strategies can the interpretation of quantitative Western blot data be relied upon to have biological relevance "
Xpert Green DNA Stain is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for in-gel staining and is compatible with both UV and Blue LED
Xpert Green DNA Stain has 2 fluorescence excitation wavelengths in the UV range (~270nm; ~290nm) and one in the blue light range (~485nm). Maximum fluorescence emission is at ~525nm (green). Therefore, Xpert Green DNA Stain is compatible with a large variety of gel documentation systems. Yellow/orange or green filters should be used for photography.
The detection limit of Xpert Green DNA Stain is in the range of 0.5-5.0 ng/band (depending on agarose type and percentage, thickness of the gel, electrophoresis buffer, transilluminator, photo camera quality and settings, etc). Band intensity can be improved by adding Xpert Green DNA Stain to the electrophoresis buffer, especially in case of small DNA fragments that migrate farther. Xpert Green DNA Stain can be used for post-staining, however, with less sensitivity.
transilluminators.
Sample -> Lysis -> Direct amplification (all in ~1h15 min)For direct amplification from whole blood, mouse tails, FTA-cards, and FFPE tissue, etc Xpert directXtract PCR kit combines a simple but efficient DNA extraction method with direct amplification using Xpert Fast Hotstart DNA polymerase in a convenient and easy-to-use manner.
This kit can be used with a variety of samples, including whole blood, mouse tails, FTA-cards, and FFPE tissue, and is thus ideal for genotyping and screening, eliminating the need of time-consuming and costly DNA purification methods.