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spl kits










pdfEasy or Advanced workflow


 See Jan explain --->


"...limitations exist in the collection and analysis of all Western blot data, regardless of the detection method ..

Tus, only with proper quality control strategies can the interpretation of quantitative Western blot data be relied upon to have biological relevance "


misleading Western


..well you should ...!

 The powerful T-Rex protein label combines excellent performance and photostability over a wide pH-range of 2-11.

It is therefore ideally suited for high performance visualization of protein samples separated in 2D gels. In contrast to green fluorescence protein stains or labels the red fluorescence of T-Rex causes almost no background.

Therefore the signal-to-noise ration of T-Rex is much better compared to most other fluorescence products on the market. 


T-Rex labeled proteins do not interfere with antibody recognition and mass spectrometry !


AdvanStainTM Scarlet – a sensitive, quantitative fluorescent stain for detecting total protein on gels and blots

AdvanStain ScarletAdvanStain Scarlet is a fluorescent stain for gels and blots that allows sensitive and quantitative visualization of proteins. Gels and blots stained with AdvanStain Scarlet can be imaged on any fluorescent imaging system, including laser- and CCD-based systems, with a detection limit of less than 1 ng of protein per band or spot.
Fully compatible with downstream Western blotting or mass spectrometry, AdvanStain Scarlet is non-toxic and biodegradable, for safe and simple disposal.


  • Sensitive - detect less than a nanogram of protein per spot
  • Convenient - simple 3-hour protocol
  • Flexible - stain gels or membranes
  • Reversible - destain for downstream Western blotting or mass spectrometry
  • No speckling - clean background and no speckling for better data
  • Safe - biodegradable and contains no heavy metals for increased safety and simple disposal
  • Compatible - image with any laser or CCD imaging system. Compatible excitation wavelengths include green (543, 532 nm), blue (488 nm), violet (405 nm) and UV (302, 365 nm). Maximum emission wavelength is 610 nm. 


AdvanStain Brochure 4pg AdvanStain Brochure 2pgAdvanStain User Manual




AdvanStain Ponceau

Rapidly detects proteins on nitrocellulose and PVDF membranes, allowing you to check the quality of protein transfer before proceeding to Western blotting. With AdvanStain Ponceau, you can quickly make sure that protein transfer has been even across the entire blot, and that no signs of bubbles or other transfer artifacts are present. The staining is reversible, and after destaining membranes can immediately be used for Western blotting.


  • Quick – detect proteins in 5 minutes or less
  • Reversible – destain in minutes
  • Compatible – after destaining, PVDF and nitrocellulose membranes are ready for Western blotting