Stain-free and standardized analysis of protein 1D gels
- Published: Friday, 04 September 2015 09:35
Quantitative and reliable protein 1D gel analysis demands a standardized and reproducible system. Proteins of samples separated by SDS- or IEF-PAGE are usually visualized using protein stains. Yet, protein stains are limited in their capabilities.
Due to the low detection sensitivity of Coomassie high amounts of sample are required. Both Coomassie and Silver stains provide a linear range of signals of only two orders of magnitude .
Fluorescent stains provide much better sensitivity and linear range, however, all stains suffer from limitations in terms of reproducibility and require additional time and labor for staining and destaining...
