GRiSP offers one of the most innovative and high performance options on the market for directPCR applications - Xpert directXtract PCR Kit.
One of the main applications for this kit (despite not limited to) is Mouse Genotyping. Not only it's an application with intensive reagent numbers consumption, but also one where time is highly relevant.
HDV is a small, defective RNA virus that can infect only individuals who have HBV; worldwide more than 15 million people are co-infected. HDV increases the severity of chronic HBV infection, frequently leading to cirrhosis, hepatic decompensation or hepatocellular carcinoma. The prevalence of HDV is declining in some endemic areas but increasing in northern and central Europe because of immigration. Interferon-alpha is currently the only available treatment option leading to suppression of HDV RNA in 25 % - 30 % of patients [
1. Not easy to organize samples. Tubes can’t stand straightly on ice
2. Ice never stops melting, and sample is easy to be contaminated when soaking in melting water
3. Ice-maker machine wastes too much energy because it has to operate every minute to maintain ice making
eCooler’s advantages:
1. Easy to organize samples, with different composition of rack
2. Stable temperature, never melt and keep samples clean
3. Save energy, diminishes 2.67MT/year CO2 emission
Replace other cooling systems, ex. dry bath
Other cooling system’s disadvantages:
1. Very slow cooling speed, usually costs more than 30 minutes from room temperature to 4 ℃
2. Only can stay in place with electricity plug hole, and can’t be moved around
3. Some cooling boxes have to be stored in refrigerator before using. Although they needn’t electricity, they occupy a lot of spaces in refrigerator and can’t make sure stable temperature after taken out
eCooler’s advantages:
1. The fastest cooling speed in the world, 10 minutes from room temperature to 4 ℃
2. Portable, temperature maintained in 4 ℃ within 30 minutes
3. No need to occupy refrigerator’s space, and promise stable temperature in ± 0.5 ℃
3. Combined with PCR
Why does PCR need eCooler?
No matter general PCR reaction or real-time PCR reaction, all reagents had better to be mixed in low temperature. dNTP and Polymerase may start wrong reaction under improper temperature, before putting tube into PCR machine. Researchers have to keep them inactive. After PCR reaction, researchers still need to keep PCR products under low temperature for following experiment.
eCooler is convenient for researchers to prepare PCR reaction solution at low temperature!
4. Easy to Freeze Cell
How eCooler helps cell-freezing?
When freezing cell, researchers have to centrifuge down cells from medium, and add freezing media to suspend. Suspending have to be operated on ice for protecting cell and them adding DMSO. Traditionally, researchers have to operate on ice, but cell is very sensitive to contamination and unstable temperature. A little mistake such as drop a piece of ice into tube could result in horrible consequences. Especially in P2/P3 cell culture room, this kind of laboratory has to stay isolation from out-world. So most of them can’t install ice-maker machine. When researchers have to use ice, they have to bring ice from out-world. It needs a complex sterilize process. Put eCooler in P2/P3 cell culture room could solve this problem. It doesn’t need to connect water pipe, doesn’t need to bring from outside, and is easy to move to liquid nitrogen barrel for next freezing step.
Improved management of hepatitis D
