Principle of the FFPE Quick RNAready kit
The FFPE Quick RNAready Kit provides special lysis and incubation conditions for optimal RNA recovery and improved template activity. Deparaffinisation of the tissue samples is followed by homogenisation and lysis by incubation with proteinase K and detergents. The lysis buffer is supplemented with the nucleic acid analogue NucleoGuard, which acts as universal inhibitor of RNases and displaces tightly bound RNA from high-molecular-weight aggregates, resulting in efficient release of RNA and preservation of the (already limited) RNA quality.
RNA solubilisation is followed by an innovative demodification step (patent pending) to reverse formalin-induced cross-links and this enables the purified RNA to deliver maximal performance as template in reverse transcription reactions and subsequent downstream assays.
FFPE_Quick_RNAready_kit_02-2011.pdf